"Investigating Electrostatic Mechanisms of Biomolecular
Organization and Function Through Vibrational Spectroscopy"
Dr. Lauren Webb
Department of Chemistry and Biochemistry
The University of Texas at Austin
Here, we describe how vibrational spectroscopy can be used to measure electrostatic fields in two complex biomolecular systems: 1) stable protein-protein interfaces 2) peptides intercalated in freestanding lipid bilayer membranes. Furthermore, we are developing computational models that accurately predict these interactions. We do this using vibrational Stark effect (VSE) spectroscopy, in which spectral shifts of a probe oscillator's energy is related directly to that probe's local electrostatic environment.